Using Mass Spectrometry To Identify and Characterize Bacteria
نویسندگان
چکیده
منابع مشابه
Using mass spectrometry to characterize self-assembled monolayers presenting peptides, proteins, and carbohydrates.
Mass spectrometry (MS) is an important technique for characterizing the structures of surfaces and has several characteristics that are especially valuable in bioanalytical applications. In biochip applications, for example, MS offers the significant advantage that it does not require analytes to be labeled–either by direct attachment of fluorescent and radioactive labels or by binding of antib...
متن کاملAnalysis of intact bacteria using rapid evaporative ionisation mass spectrometry.
An identification system for microorganisms based on recently developed rapid evaporative ionisation mass spectrometry (REIMS) is presented. Nine bacterial species cultured on various growth media were correctly identified to family-, genus-, and species-level based on their different mass spectral fingerprints using a cross-validated maximum margin criterion model.
متن کاملRecommended Mass Spectrometry-Based Strategies to Identify Ricin-Containing Samples
Ricin is a protein toxin produced by the castor bean plant (Ricinus communis) together with a related protein known as R. communis agglutinin (RCA120). Mass spectrometric (MS) assays have the capacity to unambiguously identify ricin and to detect ricin's activity in samples with complex matrices. These qualitative and quantitative assays enable detection and differentiation of ricin from the le...
متن کاملUsing stable isotope tagging and mass spectrometry to characterize protein complexes and to detect changes in their composition.
One of the primary goals of proteomics is the description of the composition, dynamics, and connections of the multiprotein modules that catalyze a wide range of biological functions in cells. Mass spectrometry (MS) has proven to be an extremely powerful tool for characterizing the composition of purified complexes. However, because MS is not a quantitative technique, the usefulness of the data...
متن کاملA Proposed Biochemical Protocol to Isolate and Characterize Acidophilic Bacteria from Tailings Soil
Indigenous acidophilic bacteria separated from mine-waste can be used in return for the addition of the reagents like sulfuric acid. Among the tailings bacteria, Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans are of the most-studied ones for the bioleaching and bioremediation of elements. In this work, the isolation and characterization of the mentioned bacteria are studied by...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Microbe Magazine
سال: 2014
ISSN: 1558-7452,1558-7460
DOI: 10.1128/microbe.9.23.1